Journal: bioRxiv
Article Title: Towards complete carbon utilization: Improved methane yield from formate and hydrogen co-feeding through constitutive formate dehydrogenase-gene expression in Methanothermobacter thermautotrophicus ΔH
doi: 10.64898/2026.03.21.713158
Figure Lengend Snippet: Gas evolution rates for M. thermautotrophicus Z-245 and M. thermautotrophicus ΔH (pFdh) during formate and H 2 co-feeding for the switch from cultivation period f to g (black dotted line). We increased the H 2 fraction in the inflow from 30 vol-% to 90 vol-%. Formate was fed at 4.16 mmol L −1 h −1 reflecting the carbon in flux (horizontal black dashed line). The sum of CO 2 an CH 4 evolution are shown in violet, and represent the formate uptake rate, disregarding biomass production. The measure formate concentration in the liquid phase is shown in Supplementary Figure S3.
Article Snippet: We obtained Methanothermobacter thermautotrophicus Z-245 (DSM 3720) and Methanothermobacter thermautotrophicus ΔH (DSM 1053) from DSMZ (Braunschweig, Germany), and the strain M. thermautotrophicus ΔH (pFdh), which is harboring the plasmid pMVS1111A:PhmtB-fdh Z-245 , from .
Techniques: Concentration Assay